Our Team
MAMBA is an innovative project aimed at understanding and combating antimicrobial resistance (AMR) in animal farming. Our objectives include identifying AMR patterns, developing effective strategies for AMR management, and promoting sustainable farming practices. By leveraging advanced metagenomic techniques, we aspire to enhance animal health and ensure food safety for communities worldwide.
This objective focuses on refining the techniques used to collect samples from animal farms that will be analyzed for antimicrobial resistance. Improvements include methods that ensure samples are representative of the farm environment, enhance the detection of AMR genes, and are efficient in terms of cost and time. The aim is to standardize sampling procedures to ensure consistency and reliability in the detection and characterization of AMR across different studies and settings.
Not all types of samples provide the same value for metagenomic analysis in the context of AMR. This objective seeks to identify which types of environmental samples (e.g., feces, dust, sewage) yield the most informative and representative data for analyzing AMR through metagenomic sequencing. Factors such as the amount of nucleic acid that can be extracted, the presence of substances that could inhibit sequencing reactions, and the overall representativeness of the samples for the farm's microbial community and AMR profile are considered.
By employing metagenomic sequencing, this objective aims to map out the presence and distribution of AMR genes across different types of farms (cattle, pig, poultry). This involves identifying the specific resistance genes present, understanding their abundance, and determining how they are spread within farm environments. Such detailed mapping can reveal hotspots of resistance and potential pathways through which resistance genes are transmitted.
Understanding where and how AMR develops and spreads within farm environments is crucial for controlling it. This objective involves analyzing the data collected to identify specific practices, conditions, or locations within farms that significantly contribute to the emergence and dissemination of AMR. Identifying these critical points can help in designing targeted interventions to mitigate the spread of AMR.
A key concern with AMR is the potential for resistance genes to transfer from animals to humans, posing significant public health risks. This objective focuses on tracing and understanding the various pathways through which humans might be exposed to AMR originating from animal farms. This includes direct contact, consumption of animal products, environmental exposure, and more.
While bacteria are the primary focus of AMR studies, resistance can also occur in other microorganisms such as fungi, helminths, ticks, and mites. This objective extends the scope of AMR surveillance to these other organisms, which are often overlooked, to gain a more comprehensive understanding of the AMR landscape in animal production environments.
Based on the findings from the research, this objective aims to develop and recommend practices that can reduce the emergence and spread of AMR on farms. This could include guidelines on the judicious use of antimicrobials, improvements in farm hygiene, biosecurity measures, and changes in animal management practices that collectively reduce the selection pressure for resistant strains.
The ultimate goal of the project is not just to gather information but to ensure that the knowledge gained is shared widely with all stakeholders involved in animal production, public health, and policy-making. This objective involves disseminating the findings through scientific publications, conferences, workshops, and online platforms, aiming to inform and influence practices and policies at both the national and international levels to better manage AMR.
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